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Arthur Kornberg (auth.), Dr. Patrick Hughes, Dr. Ellen's DNA Replication: The Regulatory Mechanisms PDF

By Arthur Kornberg (auth.), Dr. Patrick Hughes, Dr. Ellen Fanning, Dr. Masamichi Kohiyama (eds.)

DNA replication is a key occasion within the telephone cycle. even supposing our wisdom is much from entire and plenty of elusive regulatory mechanisms nonetheless stay beyondour clutch, many enzymes and a multiplicity of biochemical mechanisms concerned were came across. contemporary findings in E. coli have proven and but passed the unique speculation of F. Jacob. In yeast and better eucaryotes, the plain redundancy in putative origins and initiators has made an estimation of the significance of every pointed out aspect tricky to entry. inspite of good proven methodologies - that are additionally defined within the booklet - the foundation id in mammalian chromosomes continues to be a debatable topic. nevertheless, enormous advances were made in our knowing of virus DNA replication and this keeps to deepen and expand our knowing of the controls of mobile DNA replication.

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Res Microbiol 142: 181-188. Skarstad K, Boye E, and Steen HB (1986) Timing of initiation of chromosome replication in individual Escherichia coli cells. EMBO J 5: 1711-1717. Sotomura M, and Yoshikawa M (1975) Reinitiation of chromosome replication in the presence of chloramphenicol under an integratively suppressed state by R6K. I Bacteriol 122: 623628. Torrey TA, Atlung T, and Kogoma T (1984) dnaA suppressor (dasF) mutants of Escherichia coli are stable DNA replication (sdrA/rnh) mutants. Mol Gen Genet 196: 350-355.

Lindahl G, Hirota Y, and Jacob F (1971) On the process of cellular division in Escherichia coli: Replication of the bacterial chromosome under control of prophage P2. Proc Natl Acad Sci USA 68: 2407-2411. l derivatives: Constraints imposed by the replication terminus. J Bacteriol151: 657-667. Mao Y-M, Shi Q, Li Q-G, and Sheng Z-J (1991) recA gene dependence of replication of the Escherichia coli chromosome initiated by plasmid pUC13 integrated at predetermined sites. Mol Gen Genet 225: 234-240.

Protein synthesis was initially stimulated after a shift from 31 to 42°C, thereafter the rate of protein synthesis decreased sharply (Fig. 3). Ten min after the temperature shift the rate was at about 25% of the preshift value, and after 30 min there was hardly any protein synthesized. In contrast, in the control strain AL0152 protein synthesis first increased and was then stabilized at a higher level than before the temperature shift. Cells labelled at different times after the temperature shift were lysed and subjected to polyacrylamide gel electrophoresis.

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