By F. Hadziselimovic
Cytological recommendations have drastically more advantageous within the final two decades, mostly because of additional improvement of the microscope. Electron microscopy, specifically, has unfolded nice customers for the learn of telephone morphology, whereas the improve ment of radio-immuno-assay has introduced nice development in endocrinology. The applica tion of those thoughts, that are complementary to one another and supply at the same time aiding proof, is the topic of this monograph. The paintings is split into elements. the 1st offers with the ultrastructure of standard testicular improvement, describing 4 major parts of the testicle - the germ cells, the Sertoli cells, the peri tubular connective tissue and the Leydig mobile- with information in their person improvement. To make the electron micrographs extra simply comprehensible, diagrams were used to provide an explanation for crucial issues. the second one half offers with cryptorchid testicles, together with the first and sec ondary alterations concerned and morphometric experiences of the secondary alterations. the importance of those ultrastructural observations for the therapy of cryptorchidism is emphasised. via radio-immuno-assay the extent of testosterone in cryp torchid mice was resolute and comparisons drawn among the ultrastructural adjustments in cryptorchidism in mouse and guy. The experimental experiences served as a foundation for the speculation that, in all likelihood, a congenital disturbance of the hy pothalamo-hypophyso-gonadal axis is answerable for cryptorchidism. i'm hoping that this monograph will give a contribution in the direction of a greater realizing of ordinary and cryptorchid testicular improvement and of the etiology of cryptorchidism.
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Extra resources for Cryptorchidism: Ultrastructure of Normal and Cryptorchid Testis Development
Adjacent to the nucleus is a prominent centro somal region. The perinuclear region is relatively free of smooth endoplasmic reticulum and contains ribosomes and glycogen granules. No crystalloids are observed in mouse Leydig cells. 57 Fig. 42. Normal Leydig cell cytoplasma of adult mice. A smooth endoplasmic reticulum lies in tubular and vacuolar form in cytoplasm. P paracrystaline area, t Microbodies, M Mitochondria, t Lipoid droplets. X 20,000 Fig. 43. Leydig cells of estrogen treated adult mice.
The mitochondria are oval and of the crista type, with smooth endoplasmic reticulum, in vesicular form, lying between them. The rough endoplasmic reticulum is not so common as in the neonatal Leydig cells. It lies on the cell periphery and forms short, parallel tubes. The Golgi apparatus is composed of parallel lamellae and vesicles. It is large and found throughout the cytoplasm. 45 Fig. 31. Leydig cell of a 6-year-old normal testicle. 1 nucleus, 2 nucleolus, 3 mitochondria, 4 lipoid. X 29,000 Fig.
H-shaped junctions are to be found at 34 Fig. 23. Precursor of the lamellar body in the cytoplasm of a 13-year-old. X 95,000 intervals of approximately 660 A in the double membrane. In the transverse sections, these H-junctions appear as pores. In the spaces between the double membranes, the ground substance of the Sertoli cells is visible. Only at the junctions, where the electron-dense substance is deposited, is there no ground substance. Concentric lamellae of rough endoplasmic reticulum can be found in the cytoplasm of thirteen-year-olds 35 (Fig.