Circulating tumor cells : isolation and analysis - download pdf or read online

By Z. Hugh Fan

Introduces the reader to Circulating Tumor Cells (CTCs), their isolation process and research, and commercially on hand structures -Presents the historic point of view and the evaluation of the sphere of circulating tumor cells (CTCs) -Discusses the state-of-art tools for CTC isolation, starting from the macro- to micro-scale, from confident focus to unfavourable depletion, and from biological-property-enabled to Read more...

summary: Introduces the reader to Circulating Tumor Cells (CTCs), their isolation procedure and research, and commercially to be had structures -Presents the old viewpoint and the evaluate of the sphere of circulating tumor cells (CTCs) -Discusses the state-of-art equipment for CTC isolation, starting from the macro- to micro-scale, from confident focus to detrimental depletion, and from biological-property-enabled to physical-property-based ways -Details commercially to be had CTC systems -Describes post-isolation research and scientific translation -Provides a thesaurus of clinical phrases concerning CTCs

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The pros and cons of these are elegantly discussed by Alix-Panabières and Pantel [134] and in the following chapters of this book. It is not yet clear which approach will provide the best capture, which also will depend on specifi application needs. Another major issue is that current methods for CTC detection result in capture of tumor cells of which the majority are apoptotic [57–59]. It is important to have an alternative procedure of isolating viable CTCs that are responsible for metastases and characterizing this population.

Also, isolated DTCs can remain viable and some can be propagated in culture while maintaining the original 16 CIRCULATING TUMOR CELLS AND HISTORIC PERSPECTIVES mutational status [139, 149, 150]. They may serve as a resource for investigating new drug therapies. Recently, improved techniques for propagating CTCs in culture represent a major step forward for facilitating both in vitro and in vivo personalized drug testing [151–153]. There are already several examples of genotypic analyses that could eventually impact drug selection on individual patients.

K. (1989) Prospects for the treatment of B cell tumors using idiotypic vaccination. International Reviews of Immunology, 4, 271–310. 11. J. (1990) Anti-idiotypic therapy of leukemias and lymphomas. Chemical Immunology, 48, 126–166. 12. L. et al. (1985) Emergence of idiotype variants during treatment of B-cell lymphoma with anti-idiotype antibodies. New England Journal of Medicine, 312, 1658–1665. 13. Levy, R. A. (1990) Therapy of lymphoma directed at idiotypes. Journal of the National Cancer Institute Monographs, 10, 61–68.

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