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Download e-book for iPad: Chromatography concepts & contrasts by James M. Miller

By James M. Miller

The 1st variation of Chromatography: thoughts and Contrasts, released in 1988, was once one of many first books to debate all of the kinds of chromatography lower than one hide. the second one variation maintains with those ideas yet has been up to date to incorporate new chapters on sampling and pattern education, capillary electrophoresis and capillary electrochromatography (CEC), chromatography with mass spec detection, and commercial and governmental practices in regulated industries.

  • Covers extraction, sturdy part extraction (SPE), and stable section microextraction (SPME), and introduces mass spectrometry
  • Updated with the most recent concepts in chromatography
  • Discusses either liquid chromatography (LC)and gasoline chromatography(GC)

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First, the demand was for relatively narrow fractions of irregular silica particles, which were made by consecutive milling of larger particles and subsequent sizing. Efficient, cost-effective sizing techniques for cutting fractions at the 10 mm size range were not available at the beginning and had to be developed [6]. Second, techniques to pack these particles into stainless steel columns were known but had not been optimized to produce stable, and efficient columns. Third, it was not obvious under what conditions these columns could be operated to achieve adequate column efficiency.

Much of the current fundamental treatment of LC columns dates back to the 1960s, when chemical engineers characterized columns in unit operations, such as distillation, extraction, and adsorption, by employing such dimensionless parameters as the Reynolds, Sherwood, and Peclet numbers, respectively. In this context, the concept of the height equivalent of a theoretical plate (HETP) was developed to characterize the column performance. The HETP concept was adapted to the separation processes taking place in chromatographic columns [3,4].

Science 1989;246:64. This page intentionally left blank C H A P T E R 2 Kinetic Theories of Liquid Chromatography A. Felinger *, A. 1. 2. 1. 2. 3. 4. 5. 3. 1. 2. 3. 4. 00002-X 19 Copyright Ó 2013 Elsevier Inc. All rights reserved. 20 2. 1. INTRODUCTION Chromatographic processes are described by either equilibrium or kinetic theories. Equilibrium theories can be employed when the masstransfer and adsorptionedesorption processes are so rapid that a constant equilibrium state is established between the mobile and the stationary phases.

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