Microbiology

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Possibilities include inactivation or activation within the plasma membrane itself, or removal to or insertion from submembrane stores. The behavior of toad bladder epithelial cells in these experiments could be described adequately primarily in terms of ion conductances, with initial loss of cell chloride into gluconate medium via a chloride conductance pathway followed, as cell volume decreased, by loss of potassium conductance pathways and decreased sodium pump activity. However, in other epithelia, carrier-mediated chloride movements may be of greater importance.

Moreton, J. L. Oschman, and B. J. ), pp. 285-332. Academic Press, London. Horowitz, S. , and Miller, D. S. (1984). Solvent properties of ground substance studies by cryomicrodissection and intracellular reference-phase techniques. J. Cell Biol. 99, 172s- 179s. Hughes, P. , and Macknight, A. D. C. (1976). The regulation of cellular volume in renal cortical slices incubated in hyposmotic medium. J. Physiol. (London) 257, 137-154. Hughes, P. , and Macknight, A. D. C. (1977). Effects of replacing medium sodium by choline, ’ caesium or rubidium, on water and ion contents of renal cortical slices.

5. The effects on tissue water content of incubation, at 25°C. of renal cortical slices in sodium Ringers alone, or containing ouabain or iodoacetamide. Concentrations (mmollliter) of ouabain used were rabbit, 10, guinea pig, 10, and rat, 15. Media containing iodoacetamide ( I rnmol/liter) were gassed with nitrogen, not oxygen. Slices were all equilibrated for at least 15 min in oxygenated sodium Ringers prior to the start of the experiment. ) 24 A ANTHONY D. C. MACKNIGHT B 4 9 0 e & FIG. 6 . The behavior of rat renal cortical slices reincubated at 25°C at time zero in oxygenated sodium Ringers without (A) or with (B) ouabain (10 mmol/liter).

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