By Lawrence M. Schwartz and Barbara A. Osborne (Eds.)
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Extra resources for Cell Death
We routinely use lo6 cells per lane. The use of more cells (up to 10 ) may increase the sensitivity for detecting DNA fragments, but would only be necessary if very few cells were undergoing DNA digestion. If the required number of cells is in a volume of medium greater than 1 ml, reduce the volume by centrifuging the cells at 1000 rpm for 5 min and remove the excess medium. 5-ml Eppendorfmicrocentrifuge tube and store them on ice. Once the cells are harvested, it is important to keep them on ice suspended in either medium or phosphate buffered saline.
Hedgecock, E. , Sulston, J. , and Thompson, J. N. (1983). Mutations affecting programmed cell death in the nematode C . elegans. Science 220, 1277-1279. Kerr, J. F. , Wyllie, A. , and Currie, A. R. (1972). Apoptosis: Basic biological phenomenon with wide-ranging implication in tissue kinetics. Br. J. Cancer 26, 239-257. Modak, S. , and Bollum, F. J. (1972). Detection and measurement of single-strand breaks in nuclear DNA in fixed lens sections. Exp. Cell Res. 75, 307-313. , and Reynolds, J. D. (1991).
105, 13-20. Kerr, J. F. , Wyllie, A. , and Currie, A. R. (1972). Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J . Cancer 26, 239-257. Kerr, J. F. , Bishop, C. , and Searle, J. (1984). Apoptosis. Recenr Adv. Histopathol. 12, 1-15. Kerr, J. F. , Harmon, B. , and Bishop, C. J. (1987). Apoptosis. In Perspectives on Mammalian Cell Death (C. S. ), pp. 93-128. Oxford University Press, Oxford. Lockskin, R. , and Zakeri, Z. (1991). Programmed cell death and apoptosis.