By Leslie A. Hickle, William L. Fitch
content material: Analytical chemistry of Bacillus thuringiensis : an summary / Leslie A. Hickle and William L. Fitch --
old facets of the quantification of the lively element percent for Bacillus thuringiensis items / George Tompkins, Reto Engler, Michael Mendelsohn, and Phillip Hutton --
Bioassay tools for quantification of Bacillus thuringiensis [delta]-endotoxin / Clayton C. Beegle --
Specificity of insecticidal crystal proteins : implications for business standardization / R. Milne, A.Z. Ge, D. Rivers, and D.H. Dean --
In vitro analyses of Bacillus thuringiensis [delta]-endotoxin motion / George E. Schwab and Paul Culver --
id of entomocidal pollutants of Bacillus thuringiensis through high-performance liquid chromatography / Takashi Yamamoto --
Characterization of parasporal crystal pollution of Bacillus thuringiensis Subspecies kurstaki traces HD-1 and NRD-12 : use of oligonucleotide probes and cyanogen bromide mapping / L. Masson, M. Bossé, G. Préfontaine, L. Péloquin, P.C.K. Lau, and R. Brousseau --
improvement of a high-performance liquid chromatography assay for Bacillus thuringiensis var. san diego [delta]-endotoxin / Leonard Wittwer, Denise Colburn, Leslie A. Hickle, and T.G. Sambandan --
Use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to quantify Bacillus thuringiensis [delta]-endotoxins / Susan M. Brussock and Thomas C. Currier --
Quantitative immunoassay of insecticidal proteins in Bacillus thuringiensis items / R. Gene Groat, James W. Mattison, and Eric J. French --
The light-scattering characterization of [delta]-endotoxin creation in inclusion our bodies / Fritz S. Allen, Betty J.M. Hannoun, Tammy B. Hebner, and Kathryn Nette --
Quantification of Bacillus thuringiensis insect keep an eye on protein as expressed in transgenic crops / Roy L. Fuchs, Susan C. MacIntosh, Duff A. Dean, John T. Greenplate, Frederick J. Perlak, Jay C. Pershing, Pamela G. Marrone, and David A. Fischhoff --
High-performance liquid chromatography research of 2 [beta]-exotoxins produced by means of a few Bacillus thuringiensis lines / Barry L. Levinson.
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Additional resources for Analytical Chemistry of Bacillus thuringiensis
J. 1987, 248, 197-201. ; Vaek, M. Eur. J. Biochem. 1986, 161, 278-80. ; Pliska, V. Eur. J. Biochem. 1988, 156, 531-40. ; VanMellaert, H. Eur. J. Biochem. 1989, (in press) Wolfersberger, M. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. Chapter 5 In Vitro Analyses of Bacillus thuringiensis δ-Endotoxin Action George E. ) endotoxins as determined by in vitro experimentation. t. endotoxins. The use of cell culture and isolated tissue preparations, which comprise the primary model systems for in vitro experimentation, has certain limitations.
Thuringiensis spores 5 Q DAYS 1 Toxin 1 2 3 4 5 C r y l A a + water a a a a a 6 7 8 9 78 52 b b c c c b b c c (42-145)(31-88) C r y l A a + spores C r y l A c + water C r y l A c + spores 82 28 12 8 (43-260) (-) (6-20) (1-11) a 28 (18-48) spores only a 12 (4-21) a 8 (-) c c a 750 1800 1250 6 I) c \ ) \) C C (1-10) no m o r t a l i t y - no r e a d i n g s t a k e n on Day 2 a I n s u f f i c i e n t mortality t o calculate LD b Data t o o h e t e r o g e n o u s f o r a n a l y s i s c T e r m i n a t i o n o f a s s a y ; l a r v a b e g i n n i n g t o pupate (-) i n d i c a t e s t h a t c o n f i d e n c e l i m i t s were n o t a v a i l a b l e .
Crystal protein of B. thuringiensis aizawai (HD-249) did not display activity toward O. nubilalis despite dissolution and activation prior to ingestion (Rivers & Dean, unpublished). P. brassicae has been shown to display the same activity to crystal, protoxin, and toxin from several B. thuringiensis strains (25). This suggests that although conversion of crystal proteins to the activated toxin via a protoxin is essential for susceptibility of certain insects, other factors such as binding efficiency, proper gut receptors, composition of the protein, etc.