New PDF release: Advances in Lipid Methodology, Volume 3

By W. W. Christie

This is often the 3rd quantity of an occasional sequence of books facing elements of lipid methodology.  The participants speak about and examine positional isomers of glycerolipids, long-chain acyl-coenzyme A esters, 31P nuclear magnetic resonance profiling of phospholipids and a few very important references in lipid technique.

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Additional info for Advances in Lipid Methodology, Volume 3

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76. , in Advances in Lipid Methodology - One, pp. W. Christie, Oily Press, Ayr, Scotland). 77. , Kawabata, Y. , J. Am. Oil Chem. , 71, 475-478 ( 1994). 78. P. , Lipids, 12, 869-871 (1977). 79. , SampugnaJ. ,J. , 50, 1332-1337 (1967). 80. G. and Van Deenen,L. , Biochim. Biophys. Acta, 176, 95-100 (1969). 81. Raclot,T. ,J. , 34, 1515-1526 (1993). 82. H. ,J. Biol. , 265, 20263-20270 (1990). 83. , Fahey,J. ,J. A, 704, 99-111 (1995). 84. N. , Lipids, 5, 353-358 (1970). 85. , Ferrato,F. , Chirality, 5, 24-30 (1993).

These shift differences are particularly noticeable when analysis is attempted of extremely dilute ADVANCES IN LIPID METHODOLOGY -THREE 41 AAPC ""' EPLAS ""' JC DPG '\ DHSM DPG AAPE PA LPS )'I LAAPC LPE LEPLA~ PG LPC u GPLAS~ \ LPA- 1. 5 -1. 0 PPM Fig. 1 31 P NMR spectral phospholipid profile of a fresh water finger-form sponge, Eunapius fragilis. The top spectrum is displayed with all of the signals on scale; the bottom spectrum is the top spectrum expanded vertically to display the minor resonances: LPA, lysophosphatidic acid; GPLAS, glycerol plasmalogen; PG, phosphatidylglycerol; LEPLAS, lyso-ethanolamine plasmalogen; LPE, lysophosphatidylethanolamine; LPS, lysophosphatidylserine; PA, phosphatidic acid; AAPE, unknown resonance tentatively assigned to the alkylacylphosphatidylethanolamine; DPG, diphosphatidylglycerol; DHSM, dihydrosphingomyelin; EPLAS, ethanolamine plasmalogen; PE, phosphatidylethanolamine; PS, phosphatidylserine; U, uncharacterized phospholipid; LAAPC, lysoalkylacylphosphatidylcholine; LPC, lysophosphatidylcholine; Pl, phosphatidylinositol; AAPC, alkylacylphosphatidylcholine; PC, phosphatidylcholine.

For the purpose of signal identification, the evaporated extracted lipid sample can be treated with from 5 to 10 volumes of acetone. The phospholipids are only sparingly soluble in acetone, and by this process the acetone-insoluble phospholipids may be purged of the bulk of the neutral lipid component. 31 P NMR analysis of the acetone-insoluble phospholipid fraction will yield precise chemical-shift values that are reliable for signal identification, although, in general, these acetone-treated samples are not usable for quantitative tissue profiling.

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